Thanks to the expertise and experience of its scientific and technical staff, TLS supports researchers and companies in carrying out a wide range of experimental activities. To this end, TLS provides know-how, technological platforms, and analytical instrumentation for research activities in the biological and molecular fields, continuously expanding and strengthening its research service offering through the implementation of new platforms.
The main services and technological platforms include:
• PROTEOMICS, METABOLOMICS AND MOLECULAR PROFILING (Q-Exactive HF-X high-resolution mass spectrometer coupled with nano-flow UPLC): development of customized protocols for matrices of various origins;
• ANALYSIS OF BOTANICAL AND/OR NUTRACEUTICAL SAMPLES (Q-Exactive Plus mass spectrometer coupled with UPLC equipped with a DAD detector);
• Development of custom in vitro cellular models for toxicity, efficacy, and absorption testing of botanical and/or nutraceutical products;
• Opera Phenix HIGH CONTENT SCREENING System, a state-of-the-art instrument for high-throughput analysis, phenotype screening, and investigations on complex disease models, including live-cell experiments, primary cultures, 3D cell cultures, and microtissues;
• BSL-3 PATHOGEN CONTAINMENT LABORATORY;
• BIOINFORMATICS, providing established bioinformatics analysis approaches, recently enhanced with neural network- and AI-based data mining methods, for the development of customized pipelines for processing proteomic and metabolomic data generated through mass spectrometry;
• ANTIBODY GENERATION AND PRODUCTION
– Production and purification of recombinant proteins in mammalian cells.
– Immunogenicity studies and production of polyclonal antibodies in mice and rabbits.
– Identification and cloning of monoclonal antibodies from human, mouse, and rabbit sources using single-cell technology.
– Expression and purification of recombinant monoclonal antibodies.
– Next-generation antibody engineering (including bispecific antibodies).
– Binding assays.
– Real-time protein-protein/protein-small molecule kinetics (ka, kd, KD), concentration/titer assays (IgG), and epitope mapping.
– Developability assessment (thermal stability, colloidal stability, and application of molecular dynamics and deep learning technologies to improve binding, stability, and expression).
– In vitro functional characterization of monoclonal antibodies in BSL-2 and BSL-3 environments.
• GENE EDITING using CRISPR/Cas9 technology in cell lines and primary cells that are difficult to manipulate.
