Owing to the competencies and experience of its technical and scientific staff, the Fondazione TLS supports researchers and companies in the development of analytical methods and in carrying out in vitro experiments. To this end, TLS provides know-how, technological platforms and analytical instruments for research activities in the biological and molecular fields.
Over time, it has been possible to increasingly structure the research services offered thanks to the implementation of new technological platforms.
The main services and technological platforms comprise:
- PROTEOMICS, METABOLOMICS AND MOLECULAR PROFILING (Q-Exactive HF-X high-resolution mass spectrometer, combined with nanoflow UPLC): experimentation of custom protocols for matrices of various origins
- ANALYSIS OF SAMPLES DERIVED FROM BOTANICALS AND/OR NUTRACEUTICALS (Q-Exactive Plus mass spectrometer, coupled with UPLC fitted with a DAD detector);
- Testing of custom “in vitro” protocols for toxicity, efficacy and absorption of botanical and / or nutraceutical products
- Opera Phenix HIGH CONTENT SCREENING System. Hi-level technology for high-throughput analysis for phenotype screening and analysis on complex models of pathologies, “live” experiments on cells, primary cultures, 3D cell cultures and micro-tissues.
- BIOINFORMATICS offers established bioinformatics analysis approaches, recently enhanced with data mining methods based on neural networks and AI, for the development of customised pipelines for processing proteomic and metabolomic data derived from mass spectrometry.
- ANTIBODY GENERATION AND PRODUCTION – Production and purification of recombinant proteins in mammalian cells. – Immunogenicity studies and production of polyclonal antibodies from mice and rabbits. – Identification and cloning of monoclonal antibodies from humans, mice and rabbits using single-cell technology. – Expression and purification of recombinant monoclonal antibodies. – Next-generation antibody engineering (including bispecific antibodies) – Binding assays. – Real-time protein-protein/protein-small molecule kinetics (ka, kd, KD), concentration/titer assays (IgG), and epitope mapping. – Developability (thermal stability, colloidal stability, application of molecular dynamics and deep learning technologies to improve binding, stability and expression) – In vitro functional activity of monoclonal antibodies in BSL2 and BSL3 environments.
- Next Generation Sequencing (NGS) – (Novaseq 6000, a new-generation system for gene sequencing)
- BSL-3 – PATHOGEN CONTAINMENT LEVEL 3 LABORATORY
